Optimizing Fermentation Conditions for High Productivity of Exopolysaccharide from Bifidobacterium 22-5

doi:10.7506/spkx1002-6630-200919049

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (19 ): 214-217.doi: 10.7506/spkx1002-6630-200919049

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Optimizing Fermentation Conditions for High Productivity of Exopolysaccharide from Bifidobacterium 22-5

DING Xue-long，SHEN Qian，CHEN Qian，SHANG Nan，XU Ri-hua，LIANG Zhi-hong*

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Received:2009-08-26 Online:2009-10-01 Published:2010-12-29
Contact: LIANG Zhi-hong

Abstract

Abstract:

In order to study optimal conditions for high productivity of exopolysaccharide (EPS), fermentation experiments of Bifidobacterium 22-5 were conducted through orthogonal design based on three factors: pH of fermentation medium, temperature of fermentation medium and inoculation amount. Results indicated that optimal fermentation conditions for high productivity of EPS from Bifidobacterium 22-5 were stable pH at 7.0 for fermentation broth, 31 ℃ as the fermentation temperature and 3.0% inoculation amount. Under these optimal conditions, the productivity of EPS reached 5.8 g/L with 1.75-fold enhancement.

Key words: Bifidobacterium, exopolysaccharide, orthogonal design

CLC Number:

TQ929.2

DING Xue-long，SHEN Qian，CHEN Qian，SHANG Nan，XU Ri-hua，LIANG Zhi-hong*. Optimizing Fermentation Conditions for High Productivity of Exopolysaccharide from Bifidobacterium 22-5[J]. FOOD SCIENCE, 2009, 30(19 ): 214-217.

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Optimizing Fermentation Conditions for High Productivity of Exopolysaccharide from Bifidobacterium 22-5

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